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1Center for Immunotherapy, Vaccines and Virotherapy, The Biodesign Institute,Arizona State University, 2School of Life Sciences,Arizona State University, 3Molecular Biosciences/Biotechnology Undergraduate Program,Arizona State University, 4Department of Microbiology and Immunology,University of Michigan Medical School
Chapters
- 00:00Introduction
- 00:38Cultivate Nicotiana benthamiana Plants
- 01:53Preparation of Agrobacterium tumefaciens for Infiltration
- 03:51Transient Needle-less Agroinfiltration
- 05:24Grow and Observe the Infiltrated N.benthamiana
- 06:10Protein Extraction
- 07:56Protein G Column Chromatography
- 11:23Representative Results
- 12:56Conclusion
We describe here a simple method for expression, extraction, and purification of recombinant human IgG fused to GFP in Nicotiana benthamiana. This protocol can be extended to purification and visualization of numerous proteins that utilize column chromatography. Moreover, the protocol is adaptable to the in-person and virtual college teaching laboratory, providing project-based exploration.
