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Chapters
- 00:00Introduction
- 00:42Tissue Lysate Preparation
- 01:51Quantification and Quality Check of Tissue Lysates
- 02:33Enzymatic Digestion of Proteins
- 03:57Desalting of Digested Peptides
- 05:16Quantification of Desalted Peptides
- 05:58Labeling of Digested Peptides using iTRAQ Reagents
- 08:14Liquid Chromatography Set-up
- 09:25Mass Spectrometry Set-up
- 12:30Representative Results
- 13:45Conclusions
