This content is Free Access.
1Institute of Biomedicine, Integrative Physiology and Pharmacology Unit,University of Turku, Turku, Finland, 2Department of Pediatrics,Turku University Hospital, Turku, Finland, 3Centre for Reproductive Health,Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia, 4Australian Regenerative Medicine Institute,Monash University, Melbourne, VIC 3800, Australia
Chapters
- 00:04Introduction
- 00:51Testis Dissection and Preparation
- 02:10Transillumination-Assisted Microdissection
- 02:47Squash Preparation and Stage Verification
- 04:04Whole-Mount Seminiferous Tubule Immunostaining
- 07:04Results: Representative Mouse Seminiferous Epithelial Cycle Staging
- 09:16Conclusion
Este protocolo describe la microdisección asistida por transilluminación de segmentos de tubulos seminiferosos de ratón adultos que representan etapas específicas del ciclo epitelial seminferoso, y los tipos celulares en el mismo, y la inmunodetención posterior de preparaciones de calabaza y segmentos de tubérculos intactos.
