Journal
/
암 연구학
/
Using Computer-based Image Analysis to Improve Quantification of Lung Metastasis in the 4T1 Breast Cancer Model
JoVE 신문
암 연구학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 암 연구학
Using Computer-based Image Analysis to Improve Quantification of Lung Metastasis in the 4T1 Breast Cancer Model

Using Computer-based Image Analysis to Improve Quantification of Lung Metastasis in the 4T1 Breast Cancer Model

DOI:
10.3791/61805-v

•

08:32 min

•

October 02, 2020

•

, , , , , ,
1Department of Biomedical Sciences and Pathobiology,Virginia-Maryland College of Veterinary Medicine, 2Graduate Program in Translational Biology, Medicine and Health,Virginia Polytechnic Institute and State University, 3Department of Biomedical Engineering and Mechanics,Virginia Polytechnic Institute and State University, 4Department of Basic Science Education, Virginia Tech Carilion School of Medicine,Virginia Polytechnic Institute and State University

Chapters

  • 00:04Introduction
  • 00:51Processing Tissues
  • 02:49Staining Plates
  • 04:01Image Analysis
  • 06:26Results: Fiji-ImageJ Analysis is Accurate and Precise in Determining Metastatic Burden
  • 08:04Conclusion

Summary

자동 번역

자동 번역

We describe a more consistent and expeditious method to quantify lung metastasis in the 4T1 breast cancer model by using Fiji-ImageJ.

Tags

Computer-based Image AnalysisLung Metastasis4T1 Breast Cancer ModelQuantificationPrecisionEfficiencyBreast Cancer TherapiesCollagenaseElastaseCell Strainer6-thioguanineMethanol Fixation

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Intra-iliac Artery Injection for Efficient and Selective Modeling of Microscopic Bone Metastasis

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics

Advanced Animal Model of Colorectal Metastasis in Liver: Imaging Techniques and Properties of Metastatic Clones

Utilizing Functional Genomics Screening to Identify Potentially Novel Drug Targets in Cancer Cell Spheroid Cultures

A Portal Vein Injection Model to Study Liver Metastasis of Breast Cancer

Practical Considerations in Studying Metastatic Lung Colonization in Osteosarcoma Using the Pulmonary Metastasis Assay

Acellular and Cellular Lung Model to Study Tumor Metastasis

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Pathological Analysis of Lung Metastasis Following Lateral Tail-Vein Injection of Tumor Cells

Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System

Read Article