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在机械生物学研究的轨道摇床上的 6 井板中分段内皮细胞的生长
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Segmenting Growth of Endothelial Cells in 6-Well Plates on an Orbital Shaker for Mechanobiological Studies
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在机械生物学研究的轨道摇床上的 6 井板中分段内皮细胞的生长

DOI:
10.3791/61817-v

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12:28 min

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June 03, 2021

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, , , ,
1Department of Bioengineering,Imperial College London, 2Institute of Molecular and Cell Biology,Agency for Science, Technology and Research (A*STAR), 3Lee Kong Chian School of Medicine,Nanyang Technological University Singapore, 4Singapore Eye Research Institute,The Academia

Chapters

  • 00:00Introduction
  • 01:33Fabrication of Stainless-steel Module
  • 01:433D Printing of Polydimethylsiloxane (PDMS) Mold
  • 02:39Casting of PDMS Ring
  • 03:41Preparation of 1% Pluronic F-127
  • 04:32Coating of a 6-well Plate
  • 08:20Seeding of Endothelial Cells
  • 08:58Shear Stress Application using an Orbital Shaker
  • 09:57Representative Results
  • 11:31Conclusion

Summary

자동 번역

자동 번역

该协议描述了一种涂层方法，使用轨道摇床模型将内皮细胞生长限制在 6 井板的特定区域，用于剪切应力应用。

Tags

Endothelial Cells6-well PlatesOrbital ShakerMechanobiologyShear StressCell Growth SegmentationPDMS RingPluronic F-1273D PrintingCoating MethodCell CultureAtherosclerosis

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