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研究TGF-β信号和TGF-β引起的乳腺癌和正常细胞的上皮到中位过渡
JoVE 신문
암 연구학
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JoVE 신문 암 연구학
Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
DOI:

06:54 min

October 27, 2020

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Chapters

  • 00:05Introduction
  • 01:57Analysis of TGF-β-induced EMT
  • 04:17Results: TGF-β Signaling Responses
  • 06:20Conclusion

Summary

자동 번역

我们描述了一个系统的工作流程,通过研究这个信号通路中涉及的蛋白质和基因表达来研究TGF-β信号和TGF-β诱导的EMT。这些方法包括西方污渍、露西费拉塞记者检测、qPCR和免疫荧光染色。

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