Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Kanut Laoharawee; Matthew J. Johnson; Walker S. Lahr; Joseph J. Peterson; Beau R. Webber; Branden S. Moriarity

doi:10.3791/61855

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생물학

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Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

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JoVE 신문 생물학

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

DOI:

10.3791/61855-v

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08:20 min

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November 03, 2020

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Kanut Laoharawee^2,3, Matthew J. Johnson^2,3, Walker S. Lahr^2,3, Joseph J. Peterson^2,3, Beau R. Webber^2,3, Branden S. Moriarity^2,3

¹Department of Pediatrics,University of Minnesota, ²Center for Genomic Engineering,University of Minnesota, ³Masonic Cancer Center,University of Minnesota

Chapters

00:04Introduction
00:56Human B-cell Expansion
02:25Primary Human B-cell Engineering
05:30Results: CRISPR/Cas9-based Genome Engineering of Primary Human B Cells for Gene Knockout and Knock-in
07:33Conclusion

Summary

자동 번역

Here we provide a detailed, step-by-step protocol for CRISPR/Cas9-based genome engineering of primary human B cells for gene knockout (KO) and knock-in (KI) to study biological functions of genes in B cells and the development of B-cell therapeutics.

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

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Chapters

Summary

자동 번역

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