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Preparation of Decellularized Kidney Scaffolds in Rats
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JoVE 신문 의학
Preparation of Decellularized Kidney Scaffolds in Rats
DOI:

06:09 min

March 18, 2021

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Chapters

  • 00:04Introduction
  • 01:00Preparation for Harvesting Rat Kidneys
  • 01:55Transcardial Perfusion
  • 03:22Kidney Harvesting and Decellularization
  • 04:37Results: Rat Kidneys Subjected to Renal Arterial Perfusion Decellularization
  • 05:35Conclusion

Summary

자동 번역

This protocol introduces a method to develop a scaffold using decellularized rat kidneys. The protocol includes decellularization and recellularization processes to confirm bioavailability. Decellularization is performed using Triton X-100 and sodium dodecyl sulfate.

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