JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Department of Internal Medicine and Biomedical Research Institute,Pusan National University Hospital, 2Biomedical Research Institute,Pusan National University Hospital, 3Department of Plastic Surgery,Pusan National University Hospital, 4Department of Otorhinolaryngology – Head and Neck Surgery,Yangsan Pusan National University Hospital, 5Department of Otorhinolaryngology – Head and Neck Surgery,Pusan National University Hospital, 6Department of Pathology,Pusan National University Hospital
Chapters
- 00:04Introduction
- 01:00Preparation for Harvesting Rat Kidneys
- 01:55Transcardial Perfusion
- 03:22Kidney Harvesting and Decellularization
- 04:37Results: Rat Kidneys Subjected to Renal Arterial Perfusion Decellularization
- 05:35Conclusion
Dit protocol introduceert een methode om een steiger te ontwikkelen met behulp van gedecellulariseerde rattennieren. Het protocol omvat decellularisatie- en recellularisatieprocessen om de biologische beschikbaarheid te bevestigen. Decellularisatie wordt uitgevoerd met Triton X-100 en natriumdodecylsulfaat.
