/

의학

/

使用打开的血管窗口和活体荧光显微镜测量小鼠耳蜗中的心室血流

JoVE 신문
의학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 의학

Measurement of Strial Blood Flow in Mouse Cochlea Utilizing an Open Vessel-Window and Intravital Fluorescence Microscopy

Please note that all translations are automatically generated. Click here for the English version.

使用打开的血管窗口和活体荧光显微镜测量小鼠耳蜗中的心室血流

DOI:

10.3791/61857-v

•

09:52 min

•

September 21, 2021

•

Zhiqiang Hou, Yunpei Zhang, Lingling Neng, Jinhui Zhang*, Xiaorui Shi

¹Oregon Hearing Research Center, Department of Otolaryngology/Head & Neck Surgery,Oregon Health & Science University

Chapters

00:04Introduction
02:11Preparation of the Fluorescent-Labeled Blood Cells and Surgery to Create an Open Window
05:39Imaging of Cochlear Blood Flow Under a Fluorescence Intravital Microscope and Video Analysis
07:54Results: IVM Imaging of Cochlear Capillaries and Analysis of Change in Vessel Diameter and Blood Flow in the Stria
09:21Conclusion

Summary

자동 번역

使用荧光示踪剂的开放式血管窗口方法为耳蜗血流（CoBF）测量提供了足够的分辨率。该方法有助于研究正常和病理条件下小鼠CoBF的结构和功能变化。

Tags

Keywords: Cochlear Blood Flow Meniere’s Disease Hearing Loss Fluorescence Microscopy Transgenic Mouse Model Cardiac Puncture Blood Cell Labeling Surgical Procedure Anesthesia Vital Signs Monitoring

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

可视化和肝脏微循环血流量和耗氧量分析：急性肝炎模型中的应用

原位胶质母细胞瘤小鼠模型的维护脑实质的物理约束，并适用于活体双光子显微镜

视网膜血流小鼠活体视频显微镜测量

微循环血流速度在大鼠肺转移自动测量

在Ciliopathy鼠标突变耳蜗平面 - 细胞极性表型的评价

小鼠后肢联合活体显微镜和超声造影研究胰岛素诱导的血管舒张和肌肉灌注

活体显微镜及血栓诱导在无毛小鼠的耳垂

应用 Ameroid 的猪慢性心肌缺血模型

大鼠同时心电图记录及侵入性血压测量

通过不同的均质化方法从小鼠大脑/脊髓中检索到的多种细胞类型的同步流细胞测量特征

Read Article