Label-Free Imaging of Lipid Storage Dynamics in <em>Caenorhabditis elegans</em> using Stimulated Raman Scattering Microscopy

00:05Introduction
00:51Stimulated Raman Scattering (SRS) Microscopy Instrument Setup
03:53Imaging Condition Optimization
05:21C. elegans Sample Preparation
06:16Image Acquisition and Analysis
07:26Image Analysis
08:37Results: Representative Effects of Insulin Signaling on Lipid Storage in C. elegans
10:03Conclusion

Summary

자동 번역

Stimulated Raman scattering (SRS) microscopy allows selective, label-free imaging of specific chemical moieties and it has been effectively employed to image lipid molecules in vivo. Here, we provide a brief introduction to the principle of SRS microscopy and describe methods for its use in imaging lipid storage in Caenorhabditis elegans.

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Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy

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