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Chapters
- 00:04Introduction
- 00:38In Vitro Transcription
- 01:12Translation Material Preparation
- 01:54Liposome Preparation
- 03:07In Vitro Translation
- 05:14Proteoliposome Purification
- 05:56Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie Brilliant Blue (CBB) Staining
- 06:57Results: Representative Proteoliposome Production by Bilayer-Dialysis
- 08:01Conclusion
This protocol describes an efficient cell-free method for production of high-quality proteoliposome by bilayer-dialysis method using wheat cell-free system and liposomes. This method provides suitable means for functional analysis of membrane proteins, drug targets screening, and antibody development.
