Journal
/
/
无细胞生产蛋白脂质体,用于功能分析和靶向膜蛋白的抗体开发
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins
DOI:

08:46 min

September 22, 2020

,

Chapters

  • 00:04Introduction
  • 00:38In Vitro Transcription
  • 01:12Translation Material Preparation
  • 01:54Liposome Preparation
  • 03:07In Vitro Translation
  • 05:14Proteoliposome Purification
  • 05:56Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and Coomassie Brilliant Blue (CBB) Staining
  • 06:57Results: Representative Proteoliposome Production by Bilayer-Dialysis
  • 08:01Conclusion

Summary

자동 번역

该协议描述了一种有效的无细胞方法,通过使用小麦无细胞系统和脂质体的双层透析方法生产高质量的蛋白水产体。该方法为膜蛋白的功能分析、药物靶点筛选和抗体开发提供了合适的手段。

Related Videos

Read Article