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从人内脏脂肪组织中分离出活的脂肪细胞和基质血管组分，适用于 RNA 分析和巨噬细胞表型分析

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JoVE 신문 의학

Isolation of Viable Adipocytes and Stromal Vascular Fraction from Human Visceral Adipose Tissue Suitable for RNA Analysis and Macrophage Phenotyping

Please note that all translations are automatically generated. Click here for the English version.

从人内脏脂肪组织中分离出活的脂肪细胞和基质血管组分，适用于 RNA 分析和巨噬细胞表型分析

DOI:

10.3791/61884-v

•

06:22 min

•

October 27, 2020

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Guadalupe Estrada-Gutierrez, Eyerahi Bravo-Flores, Veronica Ortega-Castillo, Veronica Flores-Rueda, Ismael Mancilla-Herrera, Salvador Espino Y Sosa, Maribel Sánchez-Martínez, Otilia Perichart-Perera, Mario Solis-Paredes

¹Research Division,Instituto Nacional de Perinatologia, ²Department of Immunobiochemistry,Instituto Nacional de Perinatologia, ³Department of Obstetrics,Instituto Nacional de Perinatologia, ⁴Department of Academic Programs and Continuing Education,Instituto Nacional de Perinatologia, ⁵Department of Infectology and Immunology,Instituto Nacional de Perinatologia, ⁶Clinical Research Branch,Instituto Nacional de Perinatologia, ⁷Department of Nutrition and Bioprogramming,Instituto Nacional de Perinatologia, ⁸Department of Human Genetics and Genomics,Instituto Nacional de Perinatologia, Mexico City, Mexico

Chapters

00:00Introduction
00:46Visceral Adipose Tissue Collection and Enzymatic Digestion
02:10RNA Extraction from Mature Adipocytes and microRNA Quantification Assay
03:16Characterization of Macrophage Subsets from Stromal Vascular Fraction
04:42Results: Identification of Macrophages from Visceral Adipose Tissue
06:02Conclusion

Summary

자동 번역

该方案提供了一种高效的胶原酶消化方法，用于在单个过程中从人内脏脂肪中分离活脂肪细胞和基质血管部分-SVF细胞，包括从脂肪细胞中获得高质量RNA的方法，以及通过对多种膜结合标记物进行染色以通过流式细胞术分析的SVF-巨噬细胞表型。

Tags

Adipocytes Stromal Vascular Fraction RNA Analysis Macrophage Phenotyping Enzymatic Digestion Collagenase Flow Cytometry Greater Omentum

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