JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Research Division,Instituto Nacional de Perinatologia, 2Department of Immunobiochemistry,Instituto Nacional de Perinatologia, 3Department of Obstetrics,Instituto Nacional de Perinatologia, 4Department of Academic Programs and Continuing Education,Instituto Nacional de Perinatologia, 5Department of Infectology and Immunology,Instituto Nacional de Perinatologia, 6Clinical Research Branch,Instituto Nacional de Perinatologia, 7Department of Nutrition and Bioprogramming,Instituto Nacional de Perinatologia, 8Department of Human Genetics and Genomics,Instituto Nacional de Perinatologia, Mexico City, Mexico
Chapters
- 00:00Introduction
- 00:46Visceral Adipose Tissue Collection and Enzymatic Digestion
- 02:10RNA Extraction from Mature Adipocytes and microRNA Quantification Assay
- 03:16Characterization of Macrophage Subsets from Stromal Vascular Fraction
- 04:42Results: Identification of Macrophages from Visceral Adipose Tissue
- 06:02Conclusion
Dit protocol biedt een efficiënte collagenase-verteringsmethode voor isolatie van levensvatbare adipocyten en stromale vasculaire fractie-SVF-cellen uit menselijk visceraal vet in één enkel proces, inclusief methodologie om hoogwaardig RNA uit adipocyten te verkrijgen en fenotypificatie van SVF-macrofagen door kleuring van meerdere membraangebonden markers voor analyse door middel van flowcytometrie.
