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1Research Division,Instituto Nacional de Perinatologia, 2Department of Immunobiochemistry,Instituto Nacional de Perinatologia, 3Department of Obstetrics,Instituto Nacional de Perinatologia, 4Department of Academic Programs and Continuing Education,Instituto Nacional de Perinatologia, 5Department of Infectology and Immunology,Instituto Nacional de Perinatologia, 6Clinical Research Branch,Instituto Nacional de Perinatologia, 7Department of Nutrition and Bioprogramming,Instituto Nacional de Perinatologia, 8Department of Human Genetics and Genomics,Instituto Nacional de Perinatologia, Mexico City, Mexico
Chapters
- 00:00Introduction
- 00:46Visceral Adipose Tissue Collection and Enzymatic Digestion
- 02:10RNA Extraction from Mature Adipocytes and microRNA Quantification Assay
- 03:16Characterization of Macrophage Subsets from Stromal Vascular Fraction
- 04:42Results: Identification of Macrophages from Visceral Adipose Tissue
- 06:02Conclusion
Ce protocole fournit une méthode efficace de digestion de la collagénase pour isoler des adipocytes viables et des cellules stromales de fraction vasculaire-SVF de la graisse viscérale humaine en un seul processus, y compris une méthodologie pour obtenir un ARN de haute qualité à partir d’adipocytes et la phénotypification des macrophages SVF par coloration de plusieurs marqueurs membranaires pour analyse par cytométrie en flux.
