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면역학 및 감염병학

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粪便（微量）RNA分离

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Fecal (micro) RNA Isolation

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粪便（微量）RNA分离

DOI:

10.3791/61908-v

•

05:35 min

•

October 28, 2020

•

Fyonn H. Dhang², Howard L. Weiner², Shirong Liu²

¹Ann Romney Center for Neurologic Diseases, Department of Neurology, Partners Multiple Sclerosis Center,Brigham and Women’s Hospital and Harvard Medical School, ²Evergrande Center for Immunologic Diseases,Harvard Medical School and Brigham and Women’s Hospital

Chapters

00:05Introduction
00:39Feces Resuspension and Organic Extraction
01:52Final RNA Isolation
03:45Elution of the RNA with Nuclease-Free Water
04:09Results: Electrophoresis Assay and Nanodrop Analysis of Extracted RNA
05:00Conclusion

Summary

자동 번역

该协议从动物和人类受试者的粪便样本中分离出高质量的总RNA。商业 miRNA 分离试剂盒具有显著适应性，以优化数量和质量分离纯 RNA。RNA分离株适用于大多数下游RNA检测，如测序、微阵列和RT-PCR。

Tags

Keywords: Fecal MicroRNA RNA Isolation DPBS Acid Phenol Chloroform Ethanol Filter Cartridge Wash Solution

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