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胚胎注射用于CRISPR介导的诱变在蚂蚁Harpegnathos盐化剂中

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Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator

Please note that all translations are automatically generated. Click here for the English version.

胚胎注射用于CRISPR介导的诱变在蚂蚁Harpegnathos盐化剂中

DOI:

10.3791/61930-v

•

08:30 min

•

February 09, 2021

•

Kayli Sieber, Maya Saar, Comzit Opachaloemphan, Matthew Gallitto, Huan Yang, Hua Yan⁴

¹Department of Biology,University of Florida, ²Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine, ³Department of Radiation Oncology,Columbia University Medical Center, ⁴Center for Smell and Taste,University of Florida

Chapters

00:05Introduction
00:40Regular Maintenance of Harpegnathos saltator Colonies
02:05Preparation of Quartz Glass Microinjection Needles and Microinjector
04:10Injection of Embryos
06:32Results: Mutagenesis Induction in H. saltator Ants
07:37Conclusion

Summary

자동 번역

昆虫真社会性的许多特征依赖于殖民地内的交流和分工。通过显微注射和CRISPR介导的诱变对蚂蚁胚胎中关键调控基因的遗传操作为真社会昆虫利他行为的本质提供了见解。

Tags

CRISPR Mutagenesis Harpegnathos Saltator Ant Genetic Modification Embryo Injection Microinjection Plaster Nest Box Eusocial Insects 행동분석학 Communication Phenotype Plasticity Graduate Student Ant Rearing Colony Maintenance Waste Disposal Glass Microinjection Needles Casein Proteins Small Guide RNAs

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