JoVE Journal
Developmental Biology
Developmental Biology
이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.
챕터
요약
This protocol describes applications of sample immobilization using Fibrin clots, limiting drifting, and allowing the addition and washout of reagents during live imaging. Samples are transferred to a drop of Fibrinogen containing culture medium on the surface of a coverslip, after which polymerization is induced by adding thrombin.