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1Department of Medicine I, University Hospital Munich, Campus Großhadern,Ludwig-Maximilians University Munich (LMU), 2Partner Site Munich, Munich Heart Alliance (MHA),DZHK (German Centre for Cardiovascular Research), 3Walter Brendel Center of Experimental Medicine,Ludwig-Maximilians University Munich (LMU), 4Institute of Pharmacology and Toxicology,University Medical Center Göttingen, 5Partner Site Göttingen,DZHK (German Centre for Cardiovascular Research), 6Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC),University of Göttingen
Chapters
- 00:05Introduction
- 00:51Organ Harvest
- 01:33Enzymatic Digestion
- 03:59Calcium Reintroduction
- 04:57Results: Simultaneous Measurement of Calcium Currents and Transients in Atrial and Ventricular Murine Myocytes
- 05:36Conclusion
Los modelos de ratón permiten estudiar los mecanismos clave de la arritmogénesis. Para este propósito, los cardiomiocitos de alta calidad son necesarios para realizar mediciones de parche y pinza. Aquí, se describe un método para aislar miocitos aurinos y ventriculares a través de la perfusión de Langendorff retrógrada basada en enzimas, que permite mediciones simultáneas de transitorios de calcio y corriente de calcio de tipo L.
