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Neuroscience
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Preparation of Acute Slices from Dorsal Hippocampus for Whole-Cell Recording and Neuronal Reconstruction in the Dentate Gyrus of Adult Mice
 

Preparation of Acute Slices from Dorsal Hippocampus for Whole-Cell Recording and Neuronal Reconstruction in the Dentate Gyrus of Adult Mice

Article DOI: 10.3791/61980-v • 10:45 min • April 3rd, 2021
April 3rd, 2021 •
1, 1, 1
1Institute of Physiology, Christian-Albrechts-University Kiel

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요약
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We present a protocol to prepare acute-slices from the dorsal-intermediate hippocampus of mice. We compare this transversal preparation with coronal slicing in terms of quality of recordings and preservation of morphological features of recorded neurons.

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Keywords: Acute Slices Dorsal Hippocampus Whole-cell Recording Neuronal Reconstruction Dentate Gyrus Adult Mice Brain Slicing Electrophysiology Video Recordings Transversal Slices Protecting Solution Vibratome Cutting Solution Transcardial Perfusion Incubation Chamber Slice Storage Chamber
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