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半乳糖凝集素-3 - U1 snRNP复合物在珠子上的剪接活性的补充
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Complementation of Splicing Activity by a Galectin-3 – U1 snRNP Complex on Beads
DOI:

08:48 min

December 09, 2020

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Chapters

  • 00:04Introduction
  • 00:53Preparation of NE Depleted of U1 snRNP (U1ΔNE)
  • 02:10Immunoprecipitation of 10S Fractions of Glycerol Gradients by Anti-Gal3
  • 03:52Assembly of Splicing Reaction and Analysis of Products
  • 06:09Results: RNA and Polypeptide Compositions of the Anti-Gal3 Precipitate of the 10S Region of the Glycerol Gradient and its Ability to Reconstitute Splicing in U1ΔNE
  • 08:08Conclusion

Summary

자동 번역

本文描述了(a)核提取物中U1 snRNP的消耗,伴随剪接活性的丧失;(b)通过与珠子共价偶联的微乳凝集素-3 - U1 snRNP颗粒与抗半乳糖凝集素-3抗体共价结合,在U1耗尽的提取物中重建剪接活性。

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