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Chapters
- 00:04Introduction
- 00:53Preparation of NE Depleted of U1 snRNP (U1ΔNE)
- 02:10Immunoprecipitation of 10S Fractions of Glycerol Gradients by Anti-Gal3
- 03:52Assembly of Splicing Reaction and Analysis of Products
- 06:09Results: RNA and Polypeptide Compositions of the Anti-Gal3 Precipitate of the 10S Region of the Glycerol Gradient and its Ability to Reconstitute Splicing in U1ΔNE
- 08:08Conclusion
Dieser Artikel beschreibt die experimentellen Verfahren zur (a) Depletion von U1 snRNP aus Kernextrakten mit gleichzeitigem Verlust der Spleißaktivität; und (b) Rekonstitution der Spleißaktivität im U1-depletierten Extrakt durch Galectin-3 - U1-snRNP-Partikel, die an Perlen gebunden sind, kovalent gekoppelt mit Anti-Galectin-3-Antikörpern.
