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Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders
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Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders

Direct Reprogramming of Human Fibroblasts into Myoblasts to Investigate Therapies for Neuromuscular Disorders

DOI:
10.3791/61991-v

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10:28 min

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April 03, 2021

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, , , , , , ,
1Center for Gene Therapy,The Research Institute at Nationwide Children’s Hospital, 2Center for Cardiovascular Research,The Research Institute at Nationwide Children’s Hospital, 3The Heart Center,Nationwide Children’s Hospital, 4Division of Genetic and Genomic Medicine,Nationwide Children’s Hospital, 5Department of Human Genetics,The University of Utah School of Medicine, 6Department of Pediatrics,The Ohio State University

Chapters

  • 00:00Introduction
  • 00:36개요
  • 01:23Initiation of Demal Fibroblasts Culture
  • 02:46Establishment of FM Cell Lines Lentvirus-hTERT Transduction
  • 03:57Lentivirus-MyoD Transduction
  • 04:54Fibroblasts Transdifferentiation
  • 06:11Results
  • 09:16Conclusion

Summary

자동 번역

자동 번역

This protocol describes the conversion of skin fibroblasts into myoblasts and their differentiation into myotubes. The cell lines are derived from patients with neuromuscular disorders and can be used to investigate pathological mechanisms and to test therapeutic strategies.

Tags

Skin BiopsyFibroblastsMyoblastsMyotubesNeuromuscular DisordersLentivirusHTERTMYODDoxycyclineFibroblast MediumMyoblast Growth MediumDifferentiation Medium

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