Journal
/
신경과학
/
Cone-Enriched Cultures from the Retina of Chicken Embryos to Study Rod to Cone Cellular Interactions
JoVE 신문
신경과학
This content is Free Access.
JoVE 신문 신경과학
Cone-Enriched Cultures from the Retina of Chicken Embryos to Study Rod to Cone Cellular Interactions

Cone-Enriched Cultures from the Retina of Chicken Embryos to Study Rod to Cone Cellular Interactions

DOI:
10.3791/61998-v

•

08:04 min

•

March 20, 2021

•

, , , , ,
1Department of Genetics – Sorbonne Université, INSERM, CNRS,Institut de la Vision

Chapters

  • 00:04Introduction
  • 00:58Recovery of the Chicken Embryos
  • 02:11Dissection of the Retinas
  • 02:50Preparation of Retinal Cell Suspension
  • 03:40Retinal Cell Seeding
  • 05:16Results: Epithelium-Derived Cone Viability Factor (EdCVF), Clone 0073-09-37
  • 07:21Conclusion

Summary

자동 번역

자동 번역

We describe a method to obtain primary cultures of cone photoreceptors from the retina of chicken embryos and its use for high content screening.

Tags

Keywords: Cone ReceptorsChicken EmbryoRod-cone InteractionsRetinal DegenerationCone-enriched CulturePrimary Cell CultureHamburger And Hamilton StagesCarbon Dioxide-independent MediumRinger’s MediumTrypsin

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/61998?article_type=v

Derivation of Enriched Oligodendrocyte Cultures and Oligodendrocyte/Neuron Myelinating Co-cultures from Post-natal Murine Tissues
check_url/kr/61998?article_type=v

Transretinal ERG Recordings from Mouse Retina: Rod and Cone Photoresponses
check_url/kr/61998?article_type=v

Sex Stratified Neuronal Cultures to Study Ischemic Cell Death Pathways
check_url/kr/61998?article_type=v

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration
check_url/kr/61998?article_type=v

Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination
check_url/kr/61998?article_type=v

Vibratome Sectioning Mouse Retina to Prepare Photoreceptor Cultures
check_url/kr/61998?article_type=v

The Olfactory System as a Model to Study Axonal Growth Patterns and Morphology In Vivo
check_url/kr/61998?article_type=v

Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina
check_url/kr/61998?article_type=v

In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity
check_url/kr/61998?article_type=v

Time-dependent Increase in the Network Response to the Stimulation of Neuronal Cell Cultures on Micro-electrode Arrays

Read Article