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贝克酵母糖精中线粒体基因组表达产品的标记和可视化

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Labelling and Visualization of Mitochondrial Genome Expression Products in Baker’s Yeast Saccharomyces cerevisiae

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贝克酵母糖精中线粒体基因组表达产品的标记和可视化

DOI:

10.3791/62020-v

•

08:33 min

•

April 11, 2021

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Ivan V. Chicherin², Sergei A. Levitskii, Maria V. Baleva, Igor A. Krasheninnikov, Maxim V. Patrushev, Piotr A. Kamenski

¹Department of Biology,M.V. Lomonosov Moscow State University, ²Institute of Functional Genomics,M.V. Lomonosov Moscow State University, ³National Research Centre “Kurchatov Institute”

Chapters

00:00Introduction
00:39Preparation of Yeast Culture
02:10Radioactive Isotope Incorporation
03:34Yeast Cell Lysis and Extraction of Proteins
04:20Precipitation of Proteins
05:15Electrophoretic Separation
05:43Autoradiography
05:59Representative Results
07:56Conclusion

Summary

자동 번역

贝克的酵母线粒体基因组编码了八种多肽。当前协议的目标是将所有这些都标记为单独的频段，然后将其可视化为单独的频段。

Tags

Keywords: Mitochondrial Genome Mitochondrial Protein Biosynthesis Radioactive Labeling Gel Electrophoresis Saccharomyces Cerevisiae Yeast Translation Cycloheximide S35 Methionine Borymycin

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