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使用免疫荧光检测斑马鱼胚胎心脏PM2.5诱发DNA损伤
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Using Immunofluorescence to Detect PM2.5-induced DNA Damage in Zebrafish Embryo Hearts
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使用免疫荧光检测斑马鱼胚胎心脏PM2.5诱发DNA损伤

DOI:
10.3791/62021-v

•

05:58 min

•

February 15, 2021

•

, , , , , , ,
1School of Public Health,Soochow University, 2Toxicology, Risk Assessment and Research Division,Texas Commission on Environmental Quality

Chapters

  • 00:06Introduction
  • 00:34Morphological Observation of Zebrafish Embryos and Cardiac Dissection
  • 01:40Immunofluorescence Assay
  • 04:29Results: Cardiac Defects and DNA Damage of Zebrafish Embryos at 72 hpf
  • 05:36Conclusion

Summary

자동 번역

자동 번역

该协议使用免疫荧光检测检测斑马鱼胚胎解剖心脏中PM2.5诱发的DNA损伤。

Tags

ImmunofluorescencePM2.5DNA DamageZebrafish EmbryoHeart8-hydroxydeoxyguanosineGamma-H2AXAntibodyFixationImmunostainingMicroscopy

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