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使用单卵母细胞记者测定在体外成熟期间的母体 mRNA 翻译程序

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Defining the Program of Maternal mRNA Translation during In vitro Maturation using a Single Oocyte Reporter Assay

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使用单卵母细胞记者测定在体外成熟期间的母体 mRNA 翻译程序

DOI:

10.3791/62041-v

•

08:00 min

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June 16, 2021

•

Natasja G. J. Costermans², Enrico M. Daldello^2,3, Ria J. Marathe², Marco Conti²

¹Center for Reproductive Sciences,University of California at San Francisco, ²Department of Obstetrics Gynecology and Reproductive Sciences,University of California at San Francisco, ³Present affiliation: Laboratoire de Biologie du Développement-Institut de Biologie Paris Seine, LBD-IBPS,Sorbonne Université

Chapters

00:04Introduction
00:38Aspiration of Denuded Oocyte and its Microinjection
03:18Time-lapse Microscopy and Analysis of Ypet-3 UTR Translation
06:00Results: Statistical Analysis of the Expression for Reporter-YFP and mCherry in Prophase I and Maturing Oocytes
07:19Conclusion

Summary

자동 번역

该协议描述了记者在体外成熟期间研究单卵母细胞 mRNA 翻译的调节。

Tags

Keywords: Maternal MRNA Translation In Vitro Oocyte Maturation Single Oocyte Reporter Assay Time-lapse Microscopy Reporter Accumulation Translational Activation Translational Repression Antral Follicle Cumulus-oocyte Complex (COC)Denuding Cilostamide Microinjection MCherry Ypet Time-lapse Imaging

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