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Journal / Biology / 生物组织样本的串行块面扫描电子显微镜…
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生物组织样本的串行块面扫描电子显微镜 （SBF-SEM）
 
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生物组织样本的串行块面扫描电子显微镜 （SBF-SEM）

Article DOI: 10.3791/62045-v • 09:21 min • March 26th, 2021
March 26th, 2021 •
1, 1, 1, 2, 2, 1, 3,4, 1,4
1College of Optometry, University of Houston, 2Department of Biology, DePauw University, 3Center for Translational Research on Inflammatory Diseases (CTRID), Michael E. DeBakey Veterans Affairs Medical Center, 4Children’s Nutrition Center, Baylor College of Medicine

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该协议概述了使用串行块面扫描电子显微镜 （SBF-SEM） 的常规方法，这是一种强大的 3D 成像技术。SBF-SEM 的成功应用取决于适当的固定和组织染色技术，以及仔细考虑成像设置。本协议包含整个过程的实际考虑。

Tags

生物学，第169期，串行块面扫描电子显微镜，SBF-SEM，3D-EM，3D重建，组织准备，标本制备，序列部分，超结构，高分辨率，体积成像，生物，大体积
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