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Helmonteret immunfluorescensfarvning, konfokal billeddannelse og 3D-rekonstruktion af den sinoatriale og atrioventrikulære knude i musen

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JoVE Journal Medicine

Whole-Mount Immunofluorescence Staining, Confocal Imaging and 3D Reconstruction of the Sinoatrial and Atrioventricular Node in the Mouse

Helmonteret immunfluorescensfarvning, konfokal billeddannelse og 3D-rekonstruktion af den sinoatriale og atrioventrikulære knude i musen

DOI:

10.3791/62058-v

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05:16 min

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December 22, 2020

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Ruibing Xia^2,3, Julia Vlcek², Julia Bauer^2,3, Stefan Kääb³, Hellen Ishikawa-Ankerhold², Dominic Adam van den Heuvel², Christian Schulz^2,3, Steffen Massberg^2,3, Sebastian Clauss^2,3

¹University Hospital Munich, Department of Medicine I,Ludwig Maximilians University (LMU) Munich, ²Institute of Surgical Research at the Walter Brendel Center of Experimental Medicine,University Hospital Munich, Ludwig Maximilians University (LMU) Munich, ³German Center for Cardiovascular Research (DZHK), Partner Site Munich,Munich Heart Alliance

Chapters

00:04Introduction
00:51Organ Harvest and Tissue Preparation
02:23Whole-Mount Immunofluorescence Staining
03:13Confocal Microscopy
04:19Results: Reconstruction of SAN and AVN
04:49Conclusion

Summary

Automatic Translation

Vi leverer en trin-for-trin protokol til helmonteret immunfluorescensfarvning af sinoatriale knude (SAN) og atrioventrikulær knude (AVN) i murine hjerter.

Tags

Whole-mount Immunofluorescence Confocal Imaging 3D Reconstruction Sinoatrial Node Atrioventricular Node Mouse Micro-dissection PBS Paraformaldehyde Sucrose Triton X-100 Connexin 43 HTN4 Secondary Antibodies DAPI Confocal Microscopy

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