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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DOI:
10.3791/62073-v

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09:26 min

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December 29, 2021

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, ,
1Institute of Biomedical Engineering,University of Toronto

Chapters

  • 00:04Introduction
  • 00:32Oligonucleotide to Small Nuclear RNA-Activating Protein (SNAP) T7 RNA Polymerase (RNAP) Conjugation and Polyacrylamide Gel (PAGE) Analysis
  • 02:04Oligonucleotide-Tethered SNAP-T7 Purification
  • 04:13On-Demand Control Tethered RNAP Activity Demonstration
  • 07:08Results: Representative DNA-Tethered T7 RNAP Purification and Analysis
  • 08:44Conclusion

Summary

자동 번역

자동 번역

We describe the engineering of a novel DNA-tethered T7 RNA polymerase to regulate in vitro transcription reactions. We discuss the steps for protein synthesis and characterization, validate proof-of-concept transcriptional regulation, and discuss its applications in molecular computing, diagnostics, and molecular information processing.

Tags

DNA-tethered RNA PolymeraseProgrammable In Vitro TranscriptionMolecular ComputationGene Regulatory ArchitectureDNA-based CircuitsTranscriptional CircuitsSNAP T7 RNAPOligonucleotide-tethered SNAP T7 PurificationIon Exchange ColumnElution Buffer

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