The Preparation of Chicken Ex Ovo Embryos and Chorioallantoic Membrane Vessels as In Vivo Model for Contrast-Enhanced Ultrasound Imaging and Microbubble-Mediated Drug Delivery Studies

Bram Meijlink; Ilya Skachkov; Antonius F. W. van der Steen; Nico de Jong; Klazina Kooiman

doi:10.3791/62076

/

The Preparation of Chicken Ex Ovo Embryos and Chorioallantoic Membrane Vessels as In Vivo Model for Contrast-Enhanced Ultrasound Imaging and Microbubble-Mediated Drug Delivery Studies

JoVE 신문
생체공학

This content is Free Access.

JoVE 신문 생체공학

The Preparation of Chicken Ex Ovo Embryos and Chorioallantoic Membrane Vessels as In Vivo Model for Contrast-Enhanced Ultrasound Imaging and Microbubble-Mediated Drug Delivery Studies

DOI:

10.3791/62076-v

•

09:53 min

•

February 09, 2021

•

Bram Meijlink, Ilya Skachkov, Antonius F. W. van der Steen², Nico de Jong², Klazina Kooiman

¹Department of Biomedical Engineering, Thoraxcenter,Erasmus MC University Medical Center Rotterdam, ²Laboratory of Acoustical Wavefield Imaging, Faculty of Applied Sciences,Delft University of Technology

Chapters

00:05Introduction
00:43Embryo Preparation
05:24Removing Embryo and CAM from Yolk for Microscopy Imaging
07:25Results: Representative In Vivo Contrast-Enhanced Ultrasound Imaging and Microbubble-Mediated Drug Delivery
09:06Conclusion

Summary

자동 번역

This protocol describes three methods on how to obtain and use 5 to 8-day old chicken embryos and their chorioallantoic membrane (CAM) as an in vivo model to study contrast-enhanced ultrasound imaging and microbubble-mediated drug delivery.

The Preparation of Chicken Ex Ovo Embryos and Chorioallantoic Membrane Vessels as In Vivo Model for Contrast-Enhanced Ultrasound Imaging and Microbubble-Mediated Drug Delivery Studies

•

•

•

Chapters

Summary

자동 번역

Tags

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Contrast Ultrasound Targeted Treatment of Gliomas in Mice via Drug-Bearing Nanoparticle Delivery and Microvascular Ablation

Determination of the Transport Rate of Xenobiotics and Nanomaterials Across the Placenta using the ex vivo Human Placental Perfusion Model

Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides

Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall

A New Ex Vivo Model for the Evaluation of Endoscopic Submucosal Injection Material Performance

In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer

Contrast-Enhanced Subharmonic Aided Pressure Estimation (SHAPE) Using Ultrasound Imaging with a Focus on Identifying Portal Hypertension

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

Read Article