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使用多体分析在发育中的鼠标大脑中进行翻译分析

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Analysis of Translation in the Developing Mouse Brain using Polysome Profiling

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使用多体分析在发育中的鼠标大脑中进行翻译分析

DOI:

10.3791/62088-v

•

08:38 min

•

May 22, 2021

•

Shreeya Kedia³, Sarah L. Erickson³, Guang Yang^2,3

¹Department of Biochemistry and Molecular Biology; Cumming School of Medicine,University of Calgary, ²Department of Medical Genetics, Cumming School of Medicine,University of Calgary, ³Alberta Children’s Hospital Research Institute,University of Calgary

Chapters

00:04Introduction
00:50Preparation of Sucrose Gradient
01:57Tissue Dissection
03:05Cell Lysis
04:34Fractionation and Sample Collection
06:15Results: Polysome Profiling Analysis of the Developing Mouse Cortex
07:39Conclusion

Summary

자동 번역

哺乳动物大脑的发展需要在翻译水平上正确控制基因表达。在这里，我们描述了一个多体分析系统，该系统具有易于组装的蔗糖梯度制作和分馏平台，以评估 mRNA 在发育中的大脑中的转化状态。

Tags

Polysome Profiling Translational Regulation Brain Development Sucrose Gradient Embryonic Day 12 Mouse Cortex Cycloheximide RNA Extraction

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