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Celfractionering van U937-cellen door isopycnic density gradient purification
JoVE 신문
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JoVE 신문 생화학
Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification
DOI:

10:19 min

August 12, 2021

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Chapters

  • 00:04Introduction
  • 00:37Cytosolic Protein Isolation
  • 02:45Cell Homogenization
  • 03:55Debris Removal and Crude Mitochondrial and Membrane Fraction Isolation
  • 04:47Isopycnic Density Gradient Purification
  • 06:34Nuclear Protein Isolation
  • 07:57Results: Fractionation of U937 Cells by Isopycnic Density Gradient Purification
  • 09:34Conclusion

Summary

자동 번역

Dit fractioneringsprotocol stelt onderzoekers in staat om cytoplasmatische, nucleaire, mitochondriale en membraaneiwitten uit zoogdiercellen te isoleren. De laatste twee subcellulaire fracties worden verder gezuiverd via isopycnic dichtheidsgradiënt.

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