Journal
/
신경과학
/
A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

A Plate-Based Assay for the Measurement of Endogenous Monoamine Release in Acute Brain Slices

DOI:
10.3791/62127-v

•

07:56 min

•

August 11, 2021

•

, , ,
1Department of Medicine, School of Medicine,Universidad de Atacama, 2Department of Molecular, Cellular, and Biomedical Sciences,City University of New York School of Medicine at City College, 3Department of Pharmacology and Therapeutics,University of Florida College of Medicine

Chapters

  • 00:04Introduction
  • 00:44Acute Rat Brain Slice Preparation
  • 02:01Ex Vivo Endogenous Monoamine Release Induction
  • 04:42HPLC Analysis
  • 05:29Results: Representative Endogenous Monoamine Release in Acute Brain Slices
  • 07:21Conclusion

Summary

자동 번역

자동 번역

This method introduces a simple technique for the detection of endogenous monoamine release using acute brain slices. The setup uses a 48-well plate containing a tissue holder for monoamine release. Released monoamine is analyzed by HPLC coupled with electrochemical detection. Additionally, this technique provides a screening method for drug discovery.

Tags

Endogenous Monoamine ReleaseBrain SlicesPlate-based AssayDorsal StriatumHippocampusEfflux BufferPharmacological ConditionsVesicular ReleaseTransporter-mediated Release

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/62127?article_type=v

Preparation of Acute Subventricular Zone Slices for Calcium Imaging
check_url/kr/62127?article_type=v

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices
check_url/kr/62127?article_type=v

In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices
check_url/kr/62127?article_type=v

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings
check_url/kr/62127?article_type=v

Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method
check_url/kr/62127?article_type=v

In-depth Physiological Analysis of Defined Cell Populations in Acute Tissue Slices of the Mouse Vomeronasal Organ
check_url/kr/62127?article_type=v

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System
check_url/kr/62127?article_type=v

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices
check_url/kr/62127?article_type=v

Assessment of Long-term Depression Induction in Adult Cerebellar Slices
check_url/kr/62127?article_type=v

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

Read Article