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开源微型氟度计，以监测资源有限设置中的实时异热核酸放大反应

JoVE 신문
생체공학

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JoVE 신문 생체공학

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

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开源微型氟度计，以监测资源有限设置中的实时异热核酸放大反应

DOI:

10.3791/62148-v

•

09:36 min

•

February 03, 2021

•

Jackson Coole*¹, Alex Kortum*¹, Yubo Tang, Imran Vohra, Yajur Maker, Kathryn Kundrod, Mary Natoli, Rebecca Richards-Kortum

¹Department of Bioengineering,Rice University

Chapters

00:04Introduction
00:44Optical Housing Assembly
03:39Electronics and Touchscreen Assembly
06:46Real-Time Fluorescence Data Recording
08:08Results: Representative Miniature Fluorimeter Measurements
09:07Conclusion

Summary

자동 번역

提供详细的说明，以建立一个开源的模块化氟度计，与许多低成本加热器兼容，以执行实时，定量等热核酸放大。

Tags

Keyword Extraction: Open-source Miniature Fluorimeter Isothermal Nucleic Acid Amplification Resource-limited Settings Molecular Diagnostics Isothermal Amplification Optics Enclosure Test Board Focal Length Lens Long Pass Filter Emission Filter Dichroic Mirror Beam Splitter Excitation Filter LED

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