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使用CRISPR/ CasRx在 黑腹果蝇 中无处不在和组织特异性RNA靶向

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx

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使用CRISPR/ CasRx在黑腹果蝇中无处不在和组织特异性RNA靶向

DOI:

10.3791/62154-v

•

06:37 min

•

February 05, 2021

•

Ruichen Sun, Daniel Brogan, Anna Buchman, Ting Yang, Omar S. Akbari

¹Division of Biological Sciences, Section of Cell and Developmental Biology,University of California

Chapters

00:05Introduction
00:32Ubiquitous In Vivo RNA Targeting Using a Two-Component CasRx System
01:52Ubiquitous In Vivo Exogenous RNA Targeting Using a Three-Component CasRx System
04:13Luciferase Assay
04:53Results: Representative Ubiquitous and Tissue-Specific RNA Targeting in Drosophila melanogaster
06:13Conclusion

Summary

자동 번역

本文概述了在果蝇中使用RNA靶向Cas13D酶（RfxCas13D）的详细方案。

Tags

CRISPR CasRx RNA Targeting Drosophila Melanogaster Ubiquitous Gene Transcript Reduction Two-component System Three-component System GRNA Ubiq CasRx Luciferase Phenotype Mendelian Genetics

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