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Allestedsnærværende og vævsspecifik RNA-målretning i Drosophila Melanogaster ved hjælp af CRISPR/CasRx
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
DOI:

06:37 min

February 05, 2021

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Chapters

  • 00:05Introduction
  • 00:32Ubiquitous In Vivo RNA Targeting Using a Two-Component CasRx System
  • 01:52Ubiquitous In Vivo Exogenous RNA Targeting Using a Three-Component CasRx System
  • 04:13Luciferase Assay
  • 04:53Results: Representative Ubiquitous and Tissue-Specific RNA Targeting in Drosophila melanogaster
  • 06:13Conclusion

Summary

자동 번역

Denne artikel skitserer en detaljeret protokol til brug af RNA-målretning Cas13D enzym (RfxCas13D) i fluer.

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