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Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis
JoVE 신문
생물학
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JoVE 신문 생물학
Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation Analysis
DOI:

09:26 min

March 23, 2021

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Chapters

  • 00:04Introduction
  • 00:26Tissue Crosslinking
  • 02:24Chromatin Isolation
  • 03:26DNA Purification
  • 06:50Results: ChIP‐qPCR and ChIP‐Seq Analysis
  • 08:52Conclusion

Summary

자동 번역

This protocol focuses on chromatin preparation from snap frozen tissues and it is suitable for Crosslinking Chromatin Immunoprecipitation (X-ChIP) followed by either quantitative PCR analysis (X-ChIP-qPCR) or next generation sequencing approaches (X-ChIP-seq).

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