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3次元バイオリアクター培養による滑液間葉系幹細胞由来エクソソームの大規模調製
JoVE 신문
생물학
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JoVE 신문 생물학
Large-Scale Preparation of Synovial Fluid Mesenchymal Stem Cell-Derived Exosomes by 3D Bioreactor Culture
DOI:

09:48 min

July 26, 2022

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Chapters

  • 00:05Introduction
  • 00:40Human Synovial Fluid Mesenchymal Stem Cells (SF-MSCs) Culture and Identification
  • 02:42Microcarrier Preparation and Perfusion Bioreactor
  • 04:18Exosome Identification and Safety Detection
  • 05:01Nanoparticle Tracking Analysis (NTA) and Western Blotting
  • 06:48Results: Identification and Analysis of SF-MSCs and the Exosomes
  • 08:55Conclusion

Summary

자동 번역

ここでは、3Dバイオリアクターを用いて滑液間葉系幹細胞から多数のGMPグレードのエキソソームを作製するためのプロトコールを提示する。

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