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ุฌูŠู„ ู…ู† ุงู„ุฎู„ุงูŠุง ุงู„ุฌุฐุนูŠุฉ ุงู„ู…ุณุชุญุซุฉ ู…ุชุนุฏุฏุฉ ุงู„ู‚ุฏุฑุงุช ู…ู† ู…ุชู„ุงุฒู…ุฉ ุชูŠุฑู†ุฑ (45XO) ุฎู„ุงูŠุง ุงู„ุฌู†ูŠู† ู„ู†ู…ุฐุฌุฉ ุงู„ู…ุตุจ ู…ู† ุงู„ุนุฌุฒ ุงู„ุนุตุจูŠ ุงู„ู…ุฑุชุจุทุฉ ู…ุชู„ุงุฒู…ุฉ
JoVE ์‹ ๋ฌธ
๋ฐœ์ƒํ•™
Author Produced
JoVE ๋น„๋””์˜ค๋ฅผ ํ™œ์šฉํ•˜์‹œ๋ ค๋ฉด ๋„์„œ๊ด€์„ ํ†ตํ•œ ๊ธฐ๊ด€ ๊ตฌ๋…์ด ํ•„์š”ํ•ฉ๋‹ˆ๋‹ค.  ์ „์ฒด ๋น„๋””์˜ค๋ฅผ ๋ณด์‹œ๋ ค๋ฉด ๋กœ๊ทธ์ธํ•˜๊ฑฐ๋‚˜ ๋ฌด๋ฃŒ ํŠธ๋ผ์ด์–ผ์„ ์‹œ์ž‘ํ•˜์„ธ์š”.
JoVE ์‹ ๋ฌธ ๋ฐœ์ƒํ•™
Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the Syndrome
DOI:

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09:39 min

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December 04, 2021

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Chapters

  • 00:05Introduction
  • 01:12Fibroblast Isolation from Fetal Tissue
  • 02:49Plasmid Isolation and Verification
  • 03:27Nucleofection
  • 06:45iPSC Expansion
  • 07:22iPSC Characterisation
  • 07:34Differentiation to Cells of Three Germ Layers
  • 08:38Results
  • 09:16Conclusion

Summary

์ž๋™ ๋ฒˆ์—ญ

ูŠุตู ู‡ุฐุง ุงู„ุจุฑูˆุชูˆูƒูˆู„ ุชูˆู„ูŠุฏ iPSCs ุฎุงู„ูŠุฉ ู…ู† ุงู„ุชูƒุงู…ู„ ู…ู† ุงู„ุฎู„ุงูŠุง ุงู„ู„ูŠููŠุฉ ุฃู†ุณุฌุฉ ุงู„ุฌู†ูŠู† ู…ู† ุฎู„ุงู„ ุชุณู„ูŠู… ุงู„ุจู„ุงุฒู…ูŠุฏุงุช ุงู„ุธู‡ุงุฑูŠุฉ ุนู† ุทุฑูŠู‚ ุงู„ู†ูŠูˆูƒู„ูŠูˆููƒุดู† ุชู„ูŠู‡ุง ูˆุตู ุงู„ุฃุณุงู„ูŠุจ ุงู„ู…ุณุชุฎุฏู…ุฉ ู„ุชูˆุตูŠู iPSC ูˆุงู„ุชู…ุงูŠุฒ ุงู„ุนุตุจูŠ.

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