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Chapters
- 00:05Introduction
- 01:12Plasmid Construction
- 01:52Cell Culture and Transfection
- 02:41FRET Imaging
- 05:42Image Analysis for Detecting Absolute FRET Efficiencies Using Donor Quenching and Sensitized Emission
- 07:01Results: Quantification of FRET Images
- 08:28Conclusion
Förster Resonance Energy Transfer (FRET) between two fluorophore molecules can be used for studying protein interactions in the living cell. Here, a protocol is provided as to how to measure FRET in live cells by detecting sensitized emission of the acceptor and quenching of the donor molecule using confocal laser scanning microscopy.
