Journal
/
생물학
/
Particle Templated Emulsification enables Microfluidic-Free Droplet Assays
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

Particle Templated Emulsification enables Microfluidic-Free Droplet Assays

DOI:
10.3791/62248-v

•

11:03 min

•

March 09, 2021

•

, ,
1Department of Bioengineering and Therapeutic Sciences, California Institute for Quantitative Biosciences,University of California, 2Chan Zuckerberg Biohub

Chapters

  • 00:04Introduction
  • 00:44Preparation of Hydrogel Particles for Particle Templated Emulsification
  • 02:31Fabrication of the Custom Microfluidic Device
  • 04:37Fabrication of Templating Particles
  • 06:26Particle Templated Emulsification
  • 08:26Digital Droplet PCR and Analysis
  • 09:02Results: Sample Encapsulation Using Particle Templated Emulsification (PTE)
  • 10:30Conclusion

Summary

자동 번역

자동 번역

Water-in-oil droplet assays are useful for analytical chemistry, enzyme evolution, and single cell analysis, but typically require microfluidics to form the droplets. Here, we describe particle templated emulsification, a microfluidic-free approach to perform droplet assays.

Tags

Keywords: Particle Templated EmulsificationMicrofluidic-freeDroplet AssaysDigital Droplet PCREmulsificationPolyacrylamide ParticlesPhotoresistPDMSMicrofluidic FabricationPhotomaskPost Exposure BakingPGMEAIsopropanolPlasma Treatment

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/62248?article_type=v

High Speed Droplet-based Delivery System for Passive Pumping in Microfluidic Devices
check_url/kr/62248?article_type=v

Analysis of Schwann-astrocyte Interactions Using In Vitro Assays
check_url/kr/62248?article_type=v

Two Types of Assays for Detecting Frog Sperm Chemoattraction
check_url/kr/62248?article_type=v

Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae
check_url/kr/62248?article_type=v

Viability Assays for Cells in Culture
check_url/kr/62248?article_type=v

In vitro Cell Migration and Invasion Assays
check_url/kr/62248?article_type=v

Isolation and Fluorescence Imaging for Single-particle Reconstruction of Chlamydomonas Centrioles
check_url/kr/62248?article_type=v

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
check_url/kr/62248?article_type=v

Rapid Lipid Droplet Isolation Protocol Using a Well-established Organelle Isolation Kit
check_url/kr/62248?article_type=v

Dissection and Lipid Droplet Staining of Oenocytes in Drosophila Larvae

Read Article