Journal
/
/
Single Myofiber Culture Assay voor de beoordeling van volwassen spierstamcelfunctionaliteit Ex Vivo
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
DOI:

09:19 min

February 15, 2021

, , , , ,

Chapters

  • 00:04Introduction
  • 00:50Preparing Pipettes for Trituration of the Collagenase-Digested Muscles
  • 01:54EDL Muscle Isolation and Collagenase Digestion
  • 03:19Myofiber Dissociation and Culture
  • 04:20siRNA Transfection, Fixation, and IF Staining
  • 06:31Results: Culture and Transfection of Murine EDL Muscle-Derived Single Myofibres
  • 08:34Conclusion

Summary

자동 번역

In dit protocol wordt een in vitro kweek- en functionele analysemethode voor spierstamcellen beschreven, die de meeste van hun interacties met hun endogene niche behoudt.

Related Videos

Read Article