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Single Myofiber Culture Assay för bedömning av vuxna muskelstamcellsfunktionalitet Ex Vivo
JoVE 신문
생물학
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JoVE 신문 생물학
Single Myofiber Culture Assay for the Assessment of Adult Muscle Stem Cell Functionality Ex Vivo
DOI:

09:19 min

February 15, 2021

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Chapters

  • 00:04Introduction
  • 00:50Preparing Pipettes for Trituration of the Collagenase-Digested Muscles
  • 01:54EDL Muscle Isolation and Collagenase Digestion
  • 03:19Myofiber Dissociation and Culture
  • 04:20siRNA Transfection, Fixation, and IF Staining
  • 06:31Results: Culture and Transfection of Murine EDL Muscle-Derived Single Myofibres
  • 08:34Conclusion

Summary

자동 번역

I detta protokoll beskrivs en in vitro-odlings- och funktionell analysmetod för muskelstamceller, vilket bevarar de flesta av deras interaktioner med deras endogena nisch.

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