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Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

JoVE 신문
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Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes

DOI:

10.3791/62305-v

•

08:35 min

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July 17, 2021

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Meike Siebers*¹, Agatha Walla*^1,2, Thea Rütjes, Moritz-Fabian Müller, Maria von Korff^2,3

¹Institute of Plant Genetics,Heinrich-Heine-University, ²Cluster of Excellence on Plant Sciences “SMART Plants for Tomorrow’s Needs”, ³Max Planck Institute for Plant Breeding Research, ⁴IBG-1: Biotechnology, Forschungszentrum Jülich GmbH

Chapters

00:00Introduction
01:03Step 1: DNA Template Preparation
03:18Step 2: Amplification of DIS80 VNTR Locus by PCR
04:02Step 3: Agarose Gel Electrophoresis of PCR Products
05:02Step 4: Analysis of Fragment Length Results
06:05Representative Results
06:39Conclusion

Summary

자동 번역

Here we describe a simple protocol to generate DNA fingerprinting profiles by amplifying the VNTR locus D1S80 from epithelial cell DNA.

Tags

DNA Fingerprinting D1S80 Locus Variable Number Of Tandem Repeats DNA Extraction PCR Gel Electrophoresis Buccal Mucosa Epithelial Cells Lyse Solution Denaturation Buffer Isopropanol Ethanol Elution Buffer Undergraduate Lab Class

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