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评估人体骨骼肌微组织骨骼肌健康的功能指标

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Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

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评估人体骨骼肌微组织骨骼肌健康的功能指标

DOI:

10.3791/62307-v

•

09:30 min

•

February 18, 2021

•

Heta Lad², Brennen Musgrave², Majid Ebrahimi², Penney M. Gilbert^2,3

¹Institute of Biomedical Engineering,University of Toronto, ²Donnelly Centre for Cellular and Biomolecular Research,University of Toronto, ³Department of Cell and Systems Biology,University of Toronto

Chapters

00:04Introduction
00:42Preparation of Myotactic Culture Wells and Reagents for Human Skeletal Muscle Microtissues (hMMTs) Seeding
02:51Preparation of Cells for hMMTs Seeding
05:24Seeding hMMTs
07:24Results: Measurement of Contractile Force and Calcium Handling Within the Myotactic Platform
08:57Conclusion

Summary

자동 번역

本手稿描述了一种详细的方案，用于生成3D人骨骼肌微系统和微创下游功能原位测定阵列，包括收缩力和钙处理分析。

Tags

Muscle Biology Disease Modeling Candidate Molecule Testing Contractile Force Calcium Transients Longitudinal Studies Basement Membrane Extract Thrombin Fibrinogen

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