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Microcrystal Electron Diffraction of Small Molecules
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Microcrystal Electron Diffraction of Small Molecules

Microcrystal Electron Diffraction of Small Molecules

DOI:
10.3791/62313-v

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09:48 min

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March 15, 2021

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,
1Howard Hughes Medical Institute,University of California Los Angeles, 2Department of Biological Chemistry,University of California Los Angeles, 3Department of Physiology,University of California Los Angeles

Chapters

  • 00:04Introduction
  • 00:34Preparing Small Molecule Samples and TEM Grids
  • 01:37Applying Sample to Grids by Creating a Homogenous Fine Powder
  • 02:51Freezing and Loading Grids into the TEM
  • 04:12Locating and Screening Nanocrystals
  • 07:33Collecting MicroED Data
  • 08:36Results: Analysis of MicroED Dataset from Carbamazepine Microcrystal
  • 09:10Conclusion

Summary

자동 번역

자동 번역

Here, we describe the procedures developed in our laboratory for preparing powders of small molecule crystals for microcrystal electron diffraction (MicroED) experiments.

Tags

Keywords: Microcrystal Electron DiffractionSmall MoleculesPowderNanometer Size CrystalsAtomic Resolution StructuresTEM GridsGlow DischargeFilter PaperPowder TransferLiquid Nitrogen

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