Journal
/
면역학 및 감염병학
/
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions
JoVE 신문
면역학 및 감염병학
Author Produced
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

DOI:
10.3791/62324-v

•

14:58 min

•

March 05, 2022

•

, , , , ,
1Laboratory of Pathology of Infectious Diseases, Department of Pathology, Medical School,University of São Paulo, 2Scientific Platform Pasteur USP, 3Department of Clinical and Toxicological Analyses, School of Pharmaceutical Sciences,University of São Paulo, 4Hospital Israelita Albert Einstein

Chapters

  • 00:07Introduction
  • 01:15Docker Desktop Installation
  • 04:25Quality Control
  • 05:10Mapping and Annotation Steps
  • 05:51Differentially Expressed Genes Process
  • 06:10Transfer Data to the Personal Computer
  • 12:02Representative Results
  • 14:22Conclusions

Summary

자동 번역

자동 번역

The protocol presented here describes a complete pipeline to analyze RNA-sequencing transcriptome data from raw reads to functional analysis, including quality control and preprocessing steps to advanced statistical analytical approaches.

Tags

– High-throughput Transcriptome Analysis- Host-pathogen Interactions- Quality Control- Sequencing And Annotations- Statistical And Co-expression Analysis- Molecular Degree Of Perturbation Analysis- Functional Analysis- Docker Container- Chikungunya Virus- RNA Sequencing- Reference Genome- Gene Annotation

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Use of an Optical Trap for Study of Host-Pathogen Interactions for Dynamic Live Cell Imaging

Use of the EpiAirway Model for Characterizing Long-term Host-pathogen Interactions

High-throughput Detection Method for Influenza Virus

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

High-throughput Gene Tagging in Trypanosoma brucei

A New Method for Qualitative Multi-scale Analysis of Bacterial Biofilms on Filamentous Fungal Colonies Using Confocal and Electron Microscopy

Evaluation of Host-Pathogen Responses and Vaccine Efficacy in Mice

High Throughput Co-culture Assays for the Investigation of Microbial Interactions

Read Article