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Chapters
- 00:05Introduction
- 00:42Preparation of Whole Cell Lysates
- 01:43Nickel-Nitrilotriacetic Acid (Ni-NTA) Affinity Purification of Relseq and GppA
- 02:55Gel Filtration
- 03:33Synthesis of 32P-Labeled Guanosine Pentaphosphate (pppGpp) and Guanosine Tetraphosphate (ppGpp)
- 05:08DRaCALA Screening of the Target Proteins of (p)ppGpp
- 06:37Quantification and Identification of Potential Target Proteins
- 07:46Results: DRaCALA Plate Screening and Quantification
- 08:39Conclusion
